The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay

Real-time polymerase chain reaction (PCR) is the current method of choice for detection and quantification of nucleic acids, especially for molecular diagnostics. Complementarity between primers and template is often crucial for PCR applications, as mismatches can severely reduce priming efficiency....

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Detalhes bibliográficos
Main Authors: Stadhouders, Ralph, Pas, Suzan D., Anber, Jeer, Voermans, Jolanda, Mes, Ted H.M., Schutten, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2010
Assuntos:
Regular Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2797725/
https://ncbi.nlm.nih.gov/pubmed/19948821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090035
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