The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay

Real-time polymerase chain reaction (PCR) is the current method of choice for detection and quantification of nucleic acids, especially for molecular diagnostics. Complementarity between primers and template is often crucial for PCR applications, as mismatches can severely reduce priming efficiency....

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Hlavní autoři: Stadhouders, Ralph, Pas, Suzan D., Anber, Jeer, Voermans, Jolanda, Mes, Ted H.M., Schutten, Martin
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Investigative Pathology 2010
Témata:
Regular Articles
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2797725/
https://ncbi.nlm.nih.gov/pubmed/19948821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090035
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