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A directed evolution design of a GCG-specific DNA hemimethylase
DNA cytosine-5 methyltransferases (C5-MTases) are valuable models to study sequence-specific modification of DNA and are becoming increasingly important tools for biotechnology. Here we describe a structure-guided rational protein design combined with random mutagenesis and selection to change the s...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2790894/ https://ncbi.nlm.nih.gov/pubmed/19783820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp772 |
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