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A directed evolution design of a GCG-specific DNA hemimethylase

DNA cytosine-5 methyltransferases (C5-MTases) are valuable models to study sequence-specific modification of DNA and are becoming increasingly important tools for biotechnology. Here we describe a structure-guided rational protein design combined with random mutagenesis and selection to change the s...

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Sonraí Bibleagrafaíochta
Main Authors: Gerasimaitė, Rūta, Vilkaitis, Giedrius, Klimašauskas, Saulius
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2009
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790894/
https://ncbi.nlm.nih.gov/pubmed/19783820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp772
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