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Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase
Activity of the pterin- and folate-salvaging enzymes pteridine reductase 1 (PTR1) and dihydrofolate reductase–thymidylate synthetase (DHFR-TS) is commonly measured as a decrease in absorbance at 340 nm, corresponding to oxidation of nicotinamide adenine dinucleotide phosphate (NADPH). Although this...
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| Main Authors: | , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Academic Press
2010
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2789237/ https://ncbi.nlm.nih.gov/pubmed/19748480 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.09.003 |
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