Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase

Activity of the pterin- and folate-salvaging enzymes pteridine reductase 1 (PTR1) and dihydrofolate reductase–thymidylate synthetase (DHFR-TS) is commonly measured as a decrease in absorbance at 340 nm, corresponding to oxidation of nicotinamide adenine dinucleotide phosphate (NADPH). Although this...

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Главные авторы: Shanks, Emma J., Ong, Han B., Robinson, David A., Thompson, Stephen, Sienkiewicz, Natasha, Fairlamb, Alan H., Frearson, Julie A.
Формат: Artigo
Язык:Inglês
Опубликовано: Academic Press 2010
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2789237/
https://ncbi.nlm.nih.gov/pubmed/19748480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.09.003
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