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Site-directed mutagenesis, purification and assay of Saccharomyces cerevisiae RNA polymerase II
In order to analyze the structure-function of multi-subunit RNA polymerases (RNAPs), it is necessary to make site-directed mutations in key residues. Because Saccharomyces cerevisiae RNAP II is isolated as a 12 subunit enzyme that has not been amenable to in vitro reconstitution, making site-directe...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2783771/ https://ncbi.nlm.nih.gov/pubmed/19567268 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.06.016 |
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