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Use of Site-Specific Protein–DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex

A method for the photocrosslinking of proteins to DNA in purified complexes is described. It makes use of the juxtaposition of a limited number of photoreactive nucleotides with a limited number of radiolabeled nucleotides at a specific location in a DNA fragment. Protein–DNA complexes are submitted...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Forget, Diane, Domecq, Céline, Coulombe, Benoit
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4494829/
https://ncbi.nlm.nih.gov/pubmed/19378180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-015-1_26
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