Photo-Cross-Linking of a Purified Preinitiation Complex Reveals Central Roles for the RNA Polymerase II Mobile Clamp and TFIIE in Initiation Mechanisms

The topological organization of a TATA binding protein-TFIIB-TFIIF-RNA polymerase II (RNAP II)-TFIIE-promoter complex was analyzed using site-specific protein-DNA photo-cross-linking of gel-purified complexes. The cross-linking results for the subunits of RNAP II were used to determine the path of p...

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Detalhes bibliográficos
Main Authors: Forget, Diane, Langelier, Marie-France, Thérien, Cynthia, Trinh, Vincent, Coulombe, Benoit
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Transcriptional Regulation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC321454/
https://ncbi.nlm.nih.gov/pubmed/14729958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.24.3.1122-1131.2004
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