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Photo-Cross-Linking of a Purified Preinitiation Complex Reveals Central Roles for the RNA Polymerase II Mobile Clamp and TFIIE in Initiation Mechanisms

The topological organization of a TATA binding protein-TFIIB-TFIIF-RNA polymerase II (RNAP II)-TFIIE-promoter complex was analyzed using site-specific protein-DNA photo-cross-linking of gel-purified complexes. The cross-linking results for the subunits of RNAP II were used to determine the path of p...

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Udgivet i:Mol Cell Biol
Principais autores: Forget, Diane, Langelier, Marie-France, Thérien, Cynthia, Trinh, Vincent, Coulombe, Benoit
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2004
Fag:
Transcriptional Regulation
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC321454/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14729958/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/MCB.24.3.1122-1131.2004
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