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Use of Site-Specific Protein–DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex
A method for the photocrosslinking of proteins to DNA in purified complexes is described. It makes use of the juxtaposition of a limited number of photoreactive nucleotides with a limited number of radiolabeled nucleotides at a specific location in a DNA fragment. Protein–DNA complexes are submitted...
Gorde:
| Argitaratua izan da: | Methods Mol Biol |
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| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2009
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4494829/ https://ncbi.nlm.nih.gov/pubmed/19378180 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-015-1_26 |
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