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Amino acid contribution to protein solubility: Asp, Glu, and Ser contribute more favorably than the other hydrophilic amino acids in RNase Sa

Poor protein solubility is a common problem in high resolution structural studies, formulation of protein pharmaceuticals, and biochemical characterization of proteins. One popular strategy to improve protein solubility is to use site-directed mutagenesis to make hydrophobic to hydrophilic mutations...

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Autori principali: Trevino, Saul R., Scholtz, J. Martin, Pace, C. Nick
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771383/
https://ncbi.nlm.nih.gov/pubmed/17174328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2006.10.026
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