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Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa
Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W an...
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| Hauptverfasser: | , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Biophysical Society
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1304912/ https://ncbi.nlm.nih.gov/pubmed/15377518 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050377 |
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