Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa

Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Alston, Roy W., Urbanikova, Lubica, Sevcik, Jozef, Lasagna, Mauricio, Reinhart, Gregory D., Scholtz, J. Martin, Pace, C. Nick
Format: Artigo
Sprache:Inglês
Veröffentlicht: Biophysical Society 2004
Schlagworte:
Proteins
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304912/
https://ncbi.nlm.nih.gov/pubmed/15377518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050377
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge