Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa

Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W an...

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Hlavní autoři: Alston, Roy W., Urbanikova, Lubica, Sevcik, Jozef, Lasagna, Mauricio, Reinhart, Gregory D., Scholtz, J. Martin, Pace, C. Nick
Médium: Artigo
Jazyk:Inglês
Vydáno: Biophysical Society 2004
Témata:
Proteins
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304912/
https://ncbi.nlm.nih.gov/pubmed/15377518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050377
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