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Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa
Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W an...
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| 主要な著者: | , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Biophysical Society
2004
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1304912/ https://ncbi.nlm.nih.gov/pubmed/15377518 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050377 |
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