Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa

Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W an...

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Autori principali: Alston, Roy W., Urbanikova, Lubica, Sevcik, Jozef, Lasagna, Mauricio, Reinhart, Gregory D., Scholtz, J. Martin, Pace, C. Nick
Natura: Artigo
Lingua:Inglês
Pubblicazione: Biophysical Society 2004
Soggetti:
Proteins
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304912/
https://ncbi.nlm.nih.gov/pubmed/15377518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050377
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