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Tryptophan Fluorescence Reveals the Presence of Long-Range Interactions in the Denatured State of Ribonuclease Sa

Characterizing the denatured state ensemble is crucial to understanding protein stability and the mechanism of protein folding. The aim of this research was to see if fluorescence could be used to gain new information on the denatured state ensemble. Ribonuclease Sa (RNase Sa) contains no Trp residu...

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Detalhes bibliográficos
Main Authors: Alston, Roy W., Lasagna, Mauricio, Grimsley, Gerald R., Scholtz, J. Martin, Reinhart, Gregory D., Pace, C. Nick
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2257903/
https://ncbi.nlm.nih.gov/pubmed/18065473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.116954
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