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Contribution of active site residues to the activity and thermal stability of ribonuclease Sa

We have used site-specific mutagenesis to study the contribution of Glu 74 and the active site residues Gln 38, Glu 41, Glu 54, Arg 65, and His 85 to the catalytic activity and thermal stability of ribonuclease Sa. The activity of Gln38Ala is lowered by one order of magnitude, which confirms the inv...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Yakovlev, Gennady I., Mitkevich, Vladimir A., Shaw, Kevin L., Trevino, Saul, Newsom, Stephanie, Pace, C. Nick, Makarov, Alexander A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory Press 2003
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2366910/
https://ncbi.nlm.nih.gov/pubmed/14500895
Tagiau: Ychwanegu Tag
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