Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

Fluorescence-anisotropy-based homo-FRET detection methods can be employed to study clustering of identical proteins in cells. Here, the potential of fluorescence anisotropy microscopy for the quantitative imaging of protein clusters with subcellular resolution is investigated. Steady-state and time-...

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Hlavní autoři: Bader, Arjen N., Hofman, Erik G., Voortman, Jarno, van Bergen en Henegouwen, Paul M.P., Gerritsen, Hans C.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2009
Témata:
Spectroscopy, Imaging, and Other Techniques
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2770629/
https://ncbi.nlm.nih.gov/pubmed/19883605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.059
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