Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

Fluorescence-anisotropy-based homo-FRET detection methods can be employed to study clustering of identical proteins in cells. Here, the potential of fluorescence anisotropy microscopy for the quantitative imaging of protein clusters with subcellular resolution is investigated. Steady-state and time-...

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Detalles Bibliográficos
Main Authors: Bader, Arjen N., Hofman, Erik G., Voortman, Jarno, van Bergen en Henegouwen, Paul M.P., Gerritsen, Hans C.
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2009
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2770629/
https://ncbi.nlm.nih.gov/pubmed/19883605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.059
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