Defining the Limits of Single-Molecule FRET Resolution in TIRF Microscopy

Single-molecule FRET (smFRET) has long been used as a molecular ruler for the study of biology on the nanoscale (∼2–10 nm); smFRET in total-internal reflection fluorescence (TIRF) Förster resonance energy transfer (TIRF-FRET) microscopy allows multiple biomolecules to be simultaneously studied with...

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Detalhes bibliográficos
Main Authors: Holden, Seamus J., Uphoff, Stephan, Hohlbein, Johannes, Yadin, David, Le Reste, Ludovic, Britton, Oliver J., Kapanidis, Achillefs N.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965953/
https://ncbi.nlm.nih.gov/pubmed/21044609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.09.005
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