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Characterization of Dark Quencher Chromophores as Nonfluorescent Acceptors for Single-Molecule FRET

Dark quenchers are chromophores that primarily relax from the excited state to the ground state nonradiatively (i.e., are dark). As a result, they can serve as acceptors for Förster resonance energy transfer experiments without contributing significantly to background in the donor-emission channel,...

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Hlavní autoři: Le Reste, Ludovic, Hohlbein, Johannes, Gryte, Kristofer, Kapanidis, Achillefs N.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2012
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3368131/
https://ncbi.nlm.nih.gov/pubmed/22713582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.04.028
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