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Analysis of FRET Signals in the Presence of Free Donors and Acceptors()
A method for spectral analysis of Förster resonance energy transfer (FRET) signals is presented, taking into consideration both the contributions of unpaired donor and acceptor fluorophores and the influence of incomplete labeling of the interacting partners. It is shown that spectral analysis of in...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2186232/ https://ncbi.nlm.nih.gov/pubmed/17921223 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.111773 |
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