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Analysis of FRET Signals in the Presence of Free Donors and Acceptors()

A method for spectral analysis of Förster resonance energy transfer (FRET) signals is presented, taking into consideration both the contributions of unpaired donor and acceptor fluorophores and the influence of incomplete labeling of the interacting partners. It is shown that spectral analysis of in...

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Détails bibliographiques
Auteurs principaux: Wlodarczyk, Jakub, Woehler, Andrew, Kobe, Fritz, Ponimaskin, Evgeni, Zeug, Andre, Neher, Erwin
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2008
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2186232/
https://ncbi.nlm.nih.gov/pubmed/17921223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.111773
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