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Quantitative FRET Analysis by Fast Acquisition Time Domain FLIM at High Spatial Resolution in Living Cells

Quantitative analysis in Förster resonance energy transfer (FRET) experiments in live cells for protein interaction studies is still a challenging issue. In a two-component system (FRET and no FRET donor species), fitting of fluorescence lifetime imaging microscopy (FLIM) data gives the fraction of...

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Sonraí Bibleagrafaíochta
Main Authors: Padilla-Parra, Sergi, Audugé, Nicolas, Coppey-Moisan, Maïté, Tramier, Marc
Formáid: Artigo
Teanga:Inglês
Foilsithe: The Biophysical Society 2008
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2527249/
https://ncbi.nlm.nih.gov/pubmed/18539634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.131276
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