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Quantitative FRET Analysis by Fast Acquisition Time Domain FLIM at High Spatial Resolution in Living Cells

Quantitative analysis in Förster resonance energy transfer (FRET) experiments in live cells for protein interaction studies is still a challenging issue. In a two-component system (FRET and no FRET donor species), fitting of fluorescence lifetime imaging microscopy (FLIM) data gives the fraction of...

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Auteurs principaux: Padilla-Parra, Sergi, Audugé, Nicolas, Coppey-Moisan, Maïté, Tramier, Marc
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2008
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2527249/
https://ncbi.nlm.nih.gov/pubmed/18539634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.131276
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