Quantitative Comparison of Different Fluorescent Protein Couples for Fast FRET-FLIM Acquisition

The fluorescent-protein based fluorescence resonance energy transfer (FRET) approach is a powerful method for quantifying protein-protein interactions in living cells, especially when combined with fluorescence lifetime imaging microscopy (FLIM). To compare the performance of different FRET couples...

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Detalhes bibliográficos
Main Authors: Padilla-Parra, Sergi, Audugé, Nicolas, Lalucque, Hervé, Mevel, Jean-Claude, Coppey-Moisan, Maïté, Tramier, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2764072/
https://ncbi.nlm.nih.gov/pubmed/19843469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.044
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