Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

Fluorescence-anisotropy-based homo-FRET detection methods can be employed to study clustering of identical proteins in cells. Here, the potential of fluorescence anisotropy microscopy for the quantitative imaging of protein clusters with subcellular resolution is investigated. Steady-state and time-...

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Autors principals: Bader, Arjen N., Hofman, Erik G., Voortman, Jarno, van Bergen en Henegouwen, Paul M.P., Gerritsen, Hans C.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2009
Matèries:
Spectroscopy, Imaging, and Other Techniques
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2770629/
https://ncbi.nlm.nih.gov/pubmed/19883605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.059
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