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Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system

To ensure safety, regulatory agencies recommend elimination of antibiotic resistance markers from therapeutic and vaccine plasmid DNA vectors. Here, we describe the development and application of a novel antibiotic-free selection system. Vectors incorporate and express a 150 bp RNA-OUT antisense RNA...

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Detalhes bibliográficos
Main Authors: Luke, Jeremy, Carnes, Aaron E, Hodgson, Clague P, Williams, James A
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2767433/
https://ncbi.nlm.nih.gov/pubmed/19559109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vaccine.2009.06.017
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