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Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system
To ensure safety, regulatory agencies recommend elimination of antibiotic resistance markers from therapeutic and vaccine plasmid DNA vectors. Here, we describe the development and application of a novel antibiotic-free selection system. Vectors incorporate and express a 150 bp RNA-OUT antisense RNA...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2767433/ https://ncbi.nlm.nih.gov/pubmed/19559109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vaccine.2009.06.017 |
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