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Determination of binding affinity of metal cofactor to the active site of methionine aminopeptidase based on quantitation of functional enzyme
Determination of metal affinity to the active site of metalloenzymes constitutes an integral part in the understanding of enzyme catalysis and regulation. Nonlinear curve fitting of metal titration curves using the multiple independent binding sites (MIBS) model was adapted to determine K(D) values...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2009
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2760622/ https://ncbi.nlm.nih.gov/pubmed/19712663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.054 |
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