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Determination of binding affinity of metal cofactor to the active site of methionine aminopeptidase based on quantitation of functional enzyme

Determination of metal affinity to the active site of metalloenzymes constitutes an integral part in the understanding of enzyme catalysis and regulation. Nonlinear curve fitting of metal titration curves using the multiple independent binding sites (MIBS) model was adapted to determine K(D) values...

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Detalhes bibliográficos
Main Authors: Chai, Sergio C., Lu, Jing-Ping, Ye, Qi-Zhuang
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2760622/
https://ncbi.nlm.nih.gov/pubmed/19712663
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.054
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