Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis

Conventional approaches to target labelling for expression microarray analysis typically require relatively large amounts of total RNA, a serious limitation when the sample available is small. Here we explore the cycle-dependent amplification characteristics of Template-Switching PCR and validate it...

Disgrifiad llawn

Manylion Llyfryddiaeth

Cyhoeddwyd yn:	Nucleic Acids Res
Prif Awduron:	Petalidis, L., Bhattacharyya, S., Morris, G. A., Collins, V. P., Freeman, T. C., Lyons, P. A.
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Oxford University Press 2003
Pynciau:	NAR Methods Online
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602935/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng142
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!