Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis

Conventional approaches to target labelling for expression microarray analysis typically require relatively large amounts of total RNA, a serious limitation when the sample available is small. Here we explore the cycle-dependent amplification characteristics of Template-Switching PCR and validate it...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Petalidis, L., Bhattacharyya, S., Morris, G. A., Collins, V. P., Freeman, T. C., Lyons, P. A.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2003
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602935/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng142
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