Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis

Conventional approaches to target labelling for expression microarray analysis typically require relatively large amounts of total RNA, a serious limitation when the sample available is small. Here we explore the cycle-dependent amplification characteristics of Template-Switching PCR and validate it...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Petalidis, L., Bhattacharyya, S., Morris, G. A., Collins, V. P., Freeman, T. C., Lyons, P. A.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602935/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng142
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