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The effects of sequence length and oligonucleotide mismatches on 5′ exonuclease assay efficiency

Although increasingly used for DNA quantification, little is known of the dynamics of the 5′ exonuclease assay, particularly in relation to amplicon length and mismatches at oligonucleotide binding sites. In this study we used seven assays targeting the c-myc proto-oncogene to examine the effects of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Smith, Steve, Vigilant, Linda, Morin, Phillip A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2002
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC137155/
https://ncbi.nlm.nih.gov/pubmed/12384613
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