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The effects of sequence length and oligonucleotide mismatches on 5′ exonuclease assay efficiency

Although increasingly used for DNA quantification, little is known of the dynamics of the 5′ exonuclease assay, particularly in relation to amplicon length and mismatches at oligonucleotide binding sites. In this study we used seven assays targeting the c-myc proto-oncogene to examine the effects of...

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Hlavní autoři: Smith, Steve, Vigilant, Linda, Morin, Phillip A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2002
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC137155/
https://ncbi.nlm.nih.gov/pubmed/12384613
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