The effects of sequence length and oligonucleotide mismatches on 5′ exonuclease assay efficiency
Although increasingly used for DNA quantification, little is known of the dynamics of the 5′ exonuclease assay, particularly in relation to amplicon length and mismatches at oligonucleotide binding sites. In this study we used seven assays targeting the c-myc proto-oncogene to examine the effects of...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2002
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137155/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384613/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf110 |
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