Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis

Conventional approaches to target labelling for expression microarray analysis typically require relatively large amounts of total RNA, a serious limitation when the sample available is small. Here we explore the cycle-dependent amplification characteristics of Template-Switching PCR and validate it...

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Bibliografiska uppgifter
Huvudupphovsmän: Petalidis, L., Bhattacharyya, S., Morris, G. A., Collins, V. P., Freeman, T. C., Lyons, P. A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2003
Ämnen:
NAR Methods Online
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC275579/
https://ncbi.nlm.nih.gov/pubmed/14602935
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng142
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