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Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis
Conventional approaches to target labelling for expression microarray analysis typically require relatively large amounts of total RNA, a serious limitation when the sample available is small. Here we explore the cycle-dependent amplification characteristics of Template-Switching PCR and validate it...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2003
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC275579/ https://ncbi.nlm.nih.gov/pubmed/14602935 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng142 |
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