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Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers

SCRATCHY is a methodology for the construction of libraries of chimeras between genes that display low sequence homology. We have developed a strategy for library creation termed enhanced crossover SCRATCHY, that significantly increases the number of clones containing multiple crossovers. Complement...

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מידע ביבליוגרפי
Main Authors: Kawarasaki, Yasuaki, Griswold, Karl E., Stevenson, James D., Selzer, Tzvia, Benkovic, Stephen J., Iverson, Brent L., Georgiou, George
פורמט: Artigo
שפה:Inglês
יצא לאור: Oxford University Press 2003
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC275483/
https://ncbi.nlm.nih.gov/pubmed/14576326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng126
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