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Production of combinatorial libraries of fused genes by sequential transposition reactions

The use of in vivo and in vitro transposition reactions to perform non-combinatorial manipulation of DNAs in molecular biology is widespread. In this work we describe a technique that utilizes two sequential, directed transposition reactions in order to carry out combinatorial DNA manipulations. The...

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Detalhes bibliográficos
Main Authors: Naumann, Todd A., Goryshin, Igor Y., Reznikoff, William S.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC135842/
https://ncbi.nlm.nih.gov/pubmed/12409478
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