Production of combinatorial libraries of fused genes by sequential transposition reactions
The use of in vivo and in vitro transposition reactions to perform non-combinatorial manipulation of DNAs in molecular biology is widespread. In this work we describe a technique that utilizes two sequential, directed transposition reactions in order to carry out combinatorial DNA manipulations. The...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2002
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC135842/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12409478/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf118 |
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