Random multi-recombinant PCR for the construction of combinatorial protein libraries

Development of a new methodology to create protein libraries, which enable the exploration of global protein space, is an exciting challenge. In this study we have developed random multi-recombinant PCR (RM-PCR), which permits the shuffling of several DNA fragments without homologous sequences. In o...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Tsuji, Toru, Onimaru, Michiko, Yanagawa, Hiroshi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2001
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60225/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11600716/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.20.e97
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