Evolution of highly active enzymes by homology-independent recombination

The theta-class GST enzymes hGSTT1-1 (human GSTθ-1-1) and rGSTT2-2 (rat GSTθ-2-2) share 54.3% amino acid identity and exhibit different substrate specificities. Homology-independent techniques [incremental truncation for the creation of hybrid enzymes (ITCHY) and SCRATCHY] and low-homology technique...

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Hlavní autoři: Griswold, Karl E., Kawarasaki, Yasuaki, Ghoneim, Nada, Benkovic, Stephen J., Iverson, Brent L., Georgiou, George
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2005
Témata:
Biological Sciences
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177412/
https://ncbi.nlm.nih.gov/pubmed/16009931
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0504556102
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