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Evolution of highly active enzymes by homology-independent recombination
The theta-class GST enzymes hGSTT1-1 (human GSTθ-1-1) and rGSTT2-2 (rat GSTθ-2-2) share 54.3% amino acid identity and exhibit different substrate specificities. Homology-independent techniques [incremental truncation for the creation of hybrid enzymes (ITCHY) and SCRATCHY] and low-homology technique...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1177412/ https://ncbi.nlm.nih.gov/pubmed/16009931 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0504556102 |
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