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Directed evolution of protein enzymes using nonhomologous random recombination
We recently reported the development of nonhomologous random recombination (NRR) as a method for nucleic acid diversification and applied NRR to the evolution of DNA aptamers. Here, we describe a modified method, protein NRR, that enables proteins to access diversity previously difficult or impossib...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC406457/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15118093/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0402202101 |
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