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Directed evolution of protein enzymes using nonhomologous random recombination
We recently reported the development of nonhomologous random recombination (NRR) as a method for nucleic acid diversification and applied NRR to the evolution of DNA aptamers. Here, we describe a modified method, protein NRR, that enables proteins to access diversity previously difficult or impossib...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC406457/ https://ncbi.nlm.nih.gov/pubmed/15118093 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0402202101 |
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