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Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

Myriad new applications of proteases would be enabled by an ability to fine-tune substrate specificity and activity. Herein we present a general strategy for engineering protease selectivity and activity by capitalizing on sequestration of the protease to be engineered within the yeast endoplasmic r...

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Detalhes bibliográficos
Main Authors: Yi, Li, Gebhard, Mark C., Li, Qing, Taft, Joseph M., Georgiou, George, Iverson, Brent L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3645551/
https://ncbi.nlm.nih.gov/pubmed/23589865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1215994110
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