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Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
Myriad new applications of proteases would be enabled by an ability to fine-tune substrate specificity and activity. Herein we present a general strategy for engineering protease selectivity and activity by capitalizing on sequestration of the protease to be engineered within the yeast endoplasmic r...
Tallennettuna:
| Päätekijät: | , , , , , |
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| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
National Academy of Sciences
2013
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3645551/ https://ncbi.nlm.nih.gov/pubmed/23589865 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1215994110 |
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