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Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity
The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1100772/ https://ncbi.nlm.nih.gov/pubmed/15867160 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0500063102 |
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