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Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity

The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher...

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Bibliografski detalji
Glavni autori: Varadarajan, Navin, Gam, Jongsik, Olsen, Mark J., Georgiou, George, Iverson, Brent L.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1100772/
https://ncbi.nlm.nih.gov/pubmed/15867160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0500063102
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