Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport

Human concentrative nucleoside transporter, hCNT3, mediates Na(+)/nucleoside and H(+)/nucleoside co-transport. We describe a new approach to monitor H(+)/uridine co-transport in cultured mammalian cells, using a pH-sensitive monomeric red fluorescent protein variant, mNectarine, whose development an...

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Hlavní autoři: Johnson, Danielle E., Ai, Hui-wang, Wong, Peter, Young, James D., Campbell, Robert E., Casey, Joseph R.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2009
Témata:
Membrane Transport, Structure, Function, and Biogenesis
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2742814/
https://ncbi.nlm.nih.gov/pubmed/19494110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.019042
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