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Use of pH and Kinetic Isotope Effects to Establish Chemistry As Rate-Limiting in Oxidation of a Peptide Substrate by LSD1

The mechanism of oxidation of a peptide substrate by the flavoprotein lysine-specific demethylase (LSD1) has been examined using the effects of pH and isotopic substitution on steady-state and rapid-reaction kinetic parameters. The substrate contained the N-terminal 21 residues of histone H3, with a...

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Autori principali: Gaweska, Helena, Pozzi, Michelle Henderson, Schmidt, Dawn M. Z., McCafferty, Dewey G., Fitzpatrick, Paul F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2738601/
https://ncbi.nlm.nih.gov/pubmed/19408960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900499w
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