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Isotope effects suggest a stepwise mechanism for Berberine Bridge Enzyme()
The flavoprotein Berberine Bridge Enzyme (BBE) catalyzes the regioselective oxidative cyclization of (S)-reticuline to (S)-scoulerine in an alkaloid biosynthetic pathway. A series of solvent and substrate deuterium kinetic isotope effect studies were conducted in order to discriminate between a conc...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3465707/ https://ncbi.nlm.nih.gov/pubmed/22931234 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300887m |
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