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Using 3’-Bridging Phosphorothiolates to Isolate the Forward DNA Cleavage Reaction of Human Topoisomerase IIα
The ability to cleave DNA is critical to the cellular and pharmacological functions of human type II topoisomerases. However, the low level of cleavage at equilibrium and the tight coupling of the cleavage and ligation reactions make it difficult to characterize the mechanism by which these enzymes...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2008
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2733888/ https://ncbi.nlm.nih.gov/pubmed/18318502 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi702194x |
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