Unusual (1)H NMR chemical shifts support (His) C(ɛ)(1)—H⋅⋅⋅O⩵C H-bond: Proposal for reaction-driven ring flip mechanism in serine protease catalysis

(13)C-selective NMR, combined with inhibitor perturbation experiments, shows that the C(ɛ)(1)—H proton of the catalytic histidine in resting α-lytic protease and subtilisin BPN′ resonates, when protonated, at 9.22 ppm and 9.18 ppm, respectively, which is outside the normal range for such protons and...

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Main Authors: Ash, Elissa L., Sudmeier, James L., Day, Regina M., Vincent, Matthew, Torchilin, Ekaterina V., Haddad, Kristin Coffman, Bradshaw, Elizabeth M., Sanford, David G., Bachovchin, William W.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2000
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC27031/
https://ncbi.nlm.nih.gov/pubmed/10984533
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