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Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN′ by NMR

We have determined by (15)N, (1)H, and (13)C NMR, the chemical behavior of the six histidines in subtilisin BPN′ and their PMSF and peptide boronic acid complexes in aqueous solution as a function of pH in the range of from 5 to 11, and have assigned every (15)N, (1)H, C(ɛ1), and C(δ2) resonance of...

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Detaylı Bibliyografya
Asıl Yazarlar: Day, Regina M., Thalhauser, Craig J., Sudmeier, James L., Vincent, Matthew P., Torchilin, Ekaterina V., Sanford, David G., Bachovchin, Christopher W., Bachovchin, William W.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2003
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2323859/
https://ncbi.nlm.nih.gov/pubmed/12649438
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