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Versatile selection technology for intracellular protein–protein interactions mediated by a unique bacterial hitchhiker transport mechanism
We have developed a reliable genetic selection strategy for isolating interacting proteins based on the “hitchhiker” mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway. This method, designated FLI-TRAP (functional ligand-binding identification by Tat-based recognition of ass...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2656142/ https://ncbi.nlm.nih.gov/pubmed/19234130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704048106 |
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